Real-time RT-PCR demonstrates increased expression of genes bound wt p53. The bar graph depicts real-time RT-PCR detection of changes in expression in response to wt p53 overexpression. 15 selected genes whose promoters were found to be bound by wt p53 are displayed with GAPDH as a negative control. The y-axis is in logarithmic scale and displays fold change in expression relative to control cell line. The horizontal dashed line shows the relative level of expression in the control sample. The data were normalized relative to GAPDH. The error bars display the standard error of three replicates.