Figure 4

The linear regression of relative signals with relative free energies in the MPDNN method. A. Weight factors and positions of 50-mer PM probes in the MPDNN model. B. Weight factors of a MM probe with four evenly distributed mismatches. C. Linear scatter plot of relative signals and relative free energies after assigning positional weights. D. Parameters of MM dimer nucleotides in the NN and MPDNN models. The dotted line is the parameter based on a 75°C water solution based on calculations for the NN method. The solid line is the parameter obtained from the MPDNN model. E. Scatter plot of relative signals with relative free energies after the parameters of the dimer MM nucleotides were adjusted. F. The parameters of PM dimer nucleotides in the NN and MPDNN model. The dotted line is the parameter from the NN method and the solid line is the parameter observed from the MPDNN model. G. Scatter plots of relative signals with relative free energies after the parameters of dimer PM nucleotides were adjusted.