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Table 5 Evaluation of in silico (design) probes against hybridization results with RU= 1.0 and RL= 0.5 for five categories of probes

From: In silico microarray probe design for diagnosis of multiple pathogens

Category (targets) In silico Experimental
   Class A Class B Class C
I (B. mallei ATCC 23344) 0 0 (0%) 0 (0%) 0 (0%)
II (B. mallei NCTC 10229) 2 0 (0%) 2 (100%) 0 (0%)
III (B. pseudomallei K96243) 523 420 (80%) 53 (10%) 50 (10%)
IV (B. mallei ATCC 23344 and B. mallei NCTC 10229) 21 17 (81%) 2 (10%) 2 (10%)
V (B. mallei ATCC 23344, B. mallei NCTC 10229 and B. pseudomallei K96243) 431 184 (43%) 12 (3%) 236 (55%)
  1. Probes that behave as expected are categorized as Class A; i.e., these probes have normalized hybridization intensity greater than RUwith intended targets and less than RLwith non-targets. Class B probes have normalized hybridization intensity greater than RLwith non-targets, and Class C probes have normalized hybridization intensity less than RUwith the intended targets.