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Figure 6 | BMC Genomics

Figure 6

From: Experimental analysis of oligonucleotide microarray design criteria to detect deletions by comparative genomic hybridization

Figure 6

Effect of the position of a stretch of perfect identity within 50-mer oligonucleotides. LOESS regression of the difference in fluorescence intensity (in log2 scale) between the original and perturbed 50-mer oligonucleotides as a function of the length of the stretch of perfect identity. Solid (dashed) lines correspond to C. elegans (human) data. The perfect stretch of identity is either on the left (5') side (green lines), right (3') side (blue lines) or middle (red lines) of the 50-mer oligonucleotide. With NimbleGen's manufacturing process the oligonucleotides are synthesized from 3' to 5' and therefore the left side is protruding and freely floating in the solution while the right side is closer to the slide.

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BMC Genomics

ISSN: 1471-2164

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