Gene expression in spleen (A) and liver (B) analyzed with qPCR (individual samples). Data are -ΔΔCt ± SD (n = 6). Significant difference from uninfected control (t-test, p < 0.05) is indicated with *. A: spleen. Haemoglobin beta (HBB) and 5-aminolevulinate synthase (ALAS2) were selected for qPCR analyses as the representatives of genes involved in erythropoiesis and metabolism of iron. Two metalloproteinases (MMP) 9 and 13 engage in ECM remodelling and cathepsin S (CTSS) may indicate activation of the Th1-related adaptive immunity B: liver. Genes selected for qPCR analyses: heme oxygenase 1 (HMOX1) and HBB, involved in iron metabolism; beta-2-microglobulin-2 JB1 (B2M), an indicator of Th1 responses; heat shock protein 90 β (HSP90b), implied in cellular stresses; C-type mannose binding lectin (MBL1), receptor possibly involved in the recognition of SL-derived antigens. Significant difference from uninfected control (t-test, p < 0.05) is indicated with *.