Figure 4From: Local and systemic gene expression responses of Atlantic salmon (Salmo salar L.) to infection with the salmon louse (Lepeophtheirus salmonis)Gene expression in spleen (A) and liver (B) analyzed with qPCR (individual samples). Data are -ΔΔCt ± SD (n = 6). Significant difference from uninfected control (t-test, p < 0.05) is indicated with *. A: spleen. Haemoglobin beta (HBB) and 5-aminolevulinate synthase (ALAS2) were selected for qPCR analyses as the representatives of genes involved in erythropoiesis and metabolism of iron. Two metalloproteinases (MMP) 9 and 13 engage in ECM remodelling and cathepsin S (CTSS) may indicate activation of the Th1-related adaptive immunity B: liver. Genes selected for qPCR analyses: heme oxygenase 1 (HMOX1) and HBB, involved in iron metabolism; beta-2-microglobulin-2 JB1 (B2M), an indicator of Th1 responses; heat shock protein 90 β (HSP90b), implied in cellular stresses; C-type mannose binding lectin (MBL1), receptor possibly involved in the recognition of SL-derived antigens. Significant difference from uninfected control (t-test, p < 0.05) is indicated with *.Back to article page