In silico identification and molecular characterization of genes predominantly expressed in the fish oocyte

BMC Genomics

Table 1 Real-time PCR primers

Name	Acc #	Forward primer	Reverse primer
zar1	EU124662	GAACGAGCAAGGTCTACTTCAAG	GCAAGTCTGGCAGGTGATGTC
mos	EU276588	GGCGACAGGCAATATGTTTT	CACTTGGACACAATGGATCG
gdf9	EU723245	ACGAGCGACTGTGCTTTGTAC	AATGATCCAATGGCTCAGTT
btg3	EU723246	AGAGGAGGTGTGCTGCAGAT	CGTCTGAGGAGGAACAGGAG
msh4	EU723247	TCTGTCTGCGAATTCCTCCT	ACCTCCATGTGCTGGTTTTC
18S	AF308735	CGGAGGTTCGAAGACGATCA	TCGCTAGTTGGCATCGTTTAT

For all studied genes the GenBank accession number of the rainbow trout cDNA and the sequence of the primers used for the real-time PCR study are shown

ISSN: 1471-2164