Figure 5
Relationship between initial virus particle number, genome coverage and percent non-specific sequences generated by SISPA. MS2 viruses were diluted to 108, 106, 104, and 102 particles per SISPA DNAse I reaction. The sum of the total lengths of edited contigs for each dilution was calculated as percent of the total reference genome length. Non-specific sequences were determined as those sequences which did not match reference genome with a cutoff value less than 10-25.