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Table 2 Expected and observed allele sizes of conserved chicken-Charadriiformes microsatellite markers

From: Enhanced cross-species utility of conserved microsatellite markers in shorebirds

Locus Exp. allele size (bp)*       Observed allele size (bp)  
   Charadri Lari Scolopaci
   KPL OYS AVO GSH WAU CPR BSK GBT RUF RNP GSN DUN
a) standard primer design             
BmaTATC371 200 144, 152, 156 144, 146 149, 153, 157, 161, 165 152 158, 171, 173, 176, 180, 181, 190 173, 185 178, 182 173, 177, 185 140 136 failed 136
Cmms 9 252 241 241 241 245 252, 256, 258, 262 252 255 250 246 244 failed 243
Cmms 26 313 320 318 319 339 304, 308, 310 320, 327, 329, 338 304 310 316, 324 324, 326 326 312
Mopl 6 281 290, 292, 294 274 274 273 278 failed 283 267 301, 303, 305 283, 287, 291, 303 277, 293, 295 284, 288, 290, 292
Mopl 18 141 139 137 137 152 136 129 136 128 137 failed 136, 140 136
Mopl 21 230 315, 321 317 321 320 315, 322 322 322 328, 330, 334 314, 320 316, 318 322 309, 311, 313
LarsZAP26/K32 125 100, 102 98 97 94 106, 108, 133 failed 120 122, 124, 126, 134 106 99 105 106, 108
b) consensus primer design             
54F2 207 215, 217 206, 208 216 189 220 217, 221 228 219 212 212 218, 226 212
Apy07 191 146 146 144 146 170, 190, 194, 198, 202, 206 157 154 154 145 145 145 145
BmaAGGT503 255 249 248 249 247 251, 252, 253, 254 255 249 249 257 258 263 256
BmaTATC453 294 239 239 239 239 239 236 239 239, 241 239 239 237, 239, 241 239
BmaTGAA523 120 71 73 73 74 77 74 78 78 71 73 74 73
Calex-01 221 201, 209, 211, 213, 221, 227 197 200 197 201, 203 224 201 233, 235, 239, 244 207 197, 201 211 207
Calex-05 197 179, 181, 183 173 171 168 173 173, 175 173 177, 179 173 171, 173 171 173, 175
Cmms3 146 95 101 95 97 102, 106, 108, 110 93, 95, 97, 99, 101, 103 105 108 95 95 95 95
SNIPE B2 212 170, 172 183 failed 172 164 165 164 165 176, 180, 184, 196, 200 164, 168, 172, 184, 192, 196 188, 196, 200, 204, 208, 212 188
Mopl 15 170 174, 182 160 failed 161 182 178 172, 182 178 172 172 173 172
Mopl 22 404 394, 404 388 404 394 402 389 406 393 401 404 394, 404 412
Mopl 26 195 188, 190, 194, 200, 212 191 192, 194, 195 191 192, 193, 194 189, 199 188 185 184 174 184 185
Pgt 83 151 132 147 147 147 142 147 142 142 149 142, 144, 161 141, 143 142, 150, 156, 160
Rbg 18 270 259, 263, 265, 267, 269, 275, 277 262, 264 261, 265 268 260, 266, 272 Failed 268, 271 266 259, 261 265, 267 259 256, 260
Rbg 27 194 184 184 186 182 182 182 186, 188 182, 190, 196 184 186 186, 188 184
Rbg 29 129 127 125 125 127 116, 118, 126, 132, 136, 146 118, 120, 122 127 122, 132, 134, 142 127 127 120 failed
  1. The markers were tested in four unrelated individuals from a single population of each of 12 species of Charadriiformes.
  2. *expected product size in source species based on the sequence of the originally cloned allele.
  3. KPL Kentish plover (Charadrius alexandrinus), OYS oystercatcher (Haematopus ostralegus), AVO avocet (Recurvirostra avosetta), GSH greater sheathbill (Chionis alba), WAU whiskered auklet (Aethia pygmaea), CPR collared pratincole (Glareola pratincola), BSK brown skua (Catharacta lonnbergi), GBT gull-billed tern (Gelochelidon nilotica), RUF ruff (Philomachus pugnax), RNP red-necked phalarope (Phalaropus lobatus), GSN great snipe (Gallinago media), DUN dunlin (Calidris alpina)