Enhanced cross-species utility of conserved microsatellite markers in shorebirds

Küpper, Clemens; Burke, Terry; Székely, Tamás; Dawson, Deborah A

doi:10.1186/1471-2164-9-502

BMC Genomics

Table 2 Expected and observed allele sizes of conserved chicken-Charadriiformes microsatellite markers

From: Enhanced cross-species utility of conserved microsatellite markers in shorebirds

Locus	Exp. allele size (bp)*						Observed allele size (bp)
		Charadri				Lari				Scolopaci
		KPL	OYS	AVO	GSH	WAU	CPR	BSK	GBT	RUF	RNP	GSN	DUN
a) standard primer design
BmaTATC371	200	144, 152, 156	144, 146	149, 153, 157, 161, 165	152	158, 171, 173, 176, 180, 181, 190	173, 185	178, 182	173, 177, 185	140	136	failed	136
Cmms 9	252	241	241	241	245	252, 256, 258, 262	252	255	250	246	244	failed	243
Cmms 26	313	320	318	319	339	304, 308, 310	320, 327, 329, 338	304	310	316, 324	324, 326	326	312
Mopl 6	281	290, 292, 294	274	274	273	278	failed	283	267	301, 303, 305	283, 287, 291, 303	277, 293, 295	284, 288, 290, 292
Mopl 18	141	139	137	137	152	136	129	136	128	137	failed	136, 140	136
Mopl 21	230	315, 321	317	321	320	315, 322	322	322	328, 330, 334	314, 320	316, 318	322	309, 311, 313
LarsZAP26/K32	125	100, 102	98	97	94	106, 108, 133	failed	120	122, 124, 126, 134	106	99	105	106, 108
b) consensus primer design
54F2	207	215, 217	206, 208	216	189	220	217, 221	228	219	212	212	218, 226	212
Apy07	191	146	146	144	146	170, 190, 194, 198, 202, 206	157	154	154	145	145	145	145
BmaAGGT503	255	249	248	249	247	251, 252, 253, 254	255	249	249	257	258	263	256
BmaTATC453	294	239	239	239	239	239	236	239	239, 241	239	239	237, 239, 241	239
BmaTGAA523	120	71	73	73	74	77	74	78	78	71	73	74	73
Calex-01	221	201, 209, 211, 213, 221, 227	197	200	197	201, 203	224	201	233, 235, 239, 244	207	197, 201	211	207
Calex-05	197	179, 181, 183	173	171	168	173	173, 175	173	177, 179	173	171, 173	171	173, 175
Cmms3	146	95	101	95	97	102, 106, 108, 110	93, 95, 97, 99, 101, 103	105	108	95	95	95	95
SNIPE B2	212	170, 172	183	failed	172	164	165	164	165	176, 180, 184, 196, 200	164, 168, 172, 184, 192, 196	188, 196, 200, 204, 208, 212	188
Mopl 15	170	174, 182	160	failed	161	182	178	172, 182	178	172	172	173	172
Mopl 22	404	394, 404	388	404	394	402	389	406	393	401	404	394, 404	412
Mopl 26	195	188, 190, 194, 200, 212	191	192, 194, 195	191	192, 193, 194	189, 199	188	185	184	174	184	185
Pgt 83	151	132	147	147	147	142	147	142	142	149	142, 144, 161	141, 143	142, 150, 156, 160
Rbg 18	270	259, 263, 265, 267, 269, 275, 277	262, 264	261, 265	268	260, 266, 272	Failed	268, 271	266	259, 261	265, 267	259	256, 260
Rbg 27	194	184	184	186	182	182	182	186, 188	182, 190, 196	184	186	186, 188	184
Rbg 29	129	127	125	125	127	116, 118, 126, 132, 136, 146	118, 120, 122	127	122, 132, 134, 142	127	127	120	failed

The markers were tested in four unrelated individuals from a single population of each of 12 species of Charadriiformes.
*expected product size in source species based on the sequence of the originally cloned allele.
KPL Kentish plover (Charadrius alexandrinus), OYS oystercatcher (Haematopus ostralegus), AVO avocet (Recurvirostra avosetta), GSH greater sheathbill (Chionis alba), WAU whiskered auklet (Aethia pygmaea), CPR collared pratincole (Glareola pratincola), BSK brown skua (Catharacta lonnbergi), GBT gull-billed tern (Gelochelidon nilotica), RUF ruff (Philomachus pugnax), RNP red-necked phalarope (Phalaropus lobatus), GSN great snipe (Gallinago media), DUN dunlin (Calidris alpina)

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ISSN: 1471-2164

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