Figure 2

Representative two-dimensional gel electrophoresis of rainbow trout liver proteins. Total liver protein extract (150 μg) was separated first by IEF on 3–10 non-linear IPG drystrips, and then SDS-PAGE was performed on 12.5% polyacrylamide gel. The proteins were revealed by silver staining as described by Heukeshoiven and Dernick (1985). Differentially abundant proteins positively identified by trypsin digest fingerprinting are located by arrows, with their corresponding identity and spot ID.