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Table 1 Statistics of Solea senegalensis cDNA libraries

From: Genomic resources for a commercial flatfish, the Senegalese sole (Solea senegalensis): EST sequencing, oligo microarray design, and development of the Soleamold bioinformatic platform

Tissue (library)

Recombination efficiency a

Titre (pfu/ml) b

Average insert (kb)

% Representation in master library

Larvae/Juvenile

    

   Premetamorphosis

97.0

4.2 × 109

1.0

1.27

   Metamorphosis and postmetamorphosis

97.0

4.0 × 109

0.9

1.93

   Abnormal stages c

97.0

2.5 × 109

1.0

2.38

   Undifferentiated gonads d

98.4

1.8 × 108

1.4

46.10

Adult

    

   Brain (including pituitary gland)

97.3

3.0 × 109

1.2

5.35

   Stomach

97.0

6.7 × 109

1.2

0.97

   Intestine

97.0

4.1 × 109

1.0

1.88

   Liver

97.0

6.6 × 109

1.1

1.17

   Ovary e

98.2

2.0 × 108

1.0

32.68

   Testis f

97.0

0.5 × 109

1.7

6.26

  1. aThe recombination efficiency represents the percentage of clones containing inserts; bTitre after one round of amplification; cLarvae showing developmental alterations: albinism, malpigmentation, osteological malformations, and incomplete eye migration; dThree different primary libraries were pooled before amplification; eMixed tissue from ovaries at pre-vitellogenesis, vitellogenesis and maturation stages; fMixed tissue from testes at mid and late spermatogenesis, and at functional maturation stage (i.e., spermiating).
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BMC Genomics

ISSN: 1471-2164

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