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Table 2 Largest EST clusters (with ≥ 30 ESTs) in the normalized multi-tissue cDNA library of Senegalese sole

From: Genomic resources for a commercial flatfish, the Senegalese sole (Solea senegalensis): EST sequencing, oligo microarray design, and development of the Soleamold bioinformatic platform

GenBank hit acc. no a

Gene identification (species) of top BLAST hit a

BLAST

E-value a

Length

(% identity) b

No. ESTs

ABQ58114

Vitellogenin Ab (Hippoglossus hippoglossus)

1E-109

185 (78%),

150

CAC45059

Type I keratin S8 (Oncorhynchus mykiss)

1E-137

299 (83%)

136

ABQ58113

Vitellogenin Aa (Hippoglossus hippoglossus)

0

1293 (73%)

112

CAH59609

Apolipoprotein AI precursor (Platichthys flesus)

2E-70

260 (52%)

98

P68140

Actin, alpha skeletal muscle A (Takifugu rubripes)

0

377 (100%)

94

BAB12571

Myosin heavy chain (Pennahia argentata)

0

779 (95%)

82

AAY21007

Zona pellucida protein Bb (Sparus aurata)

3E-139

353 (77%)

78

BAA85157

Elongation factor 1 alpha (Seriola quinqueradiata)

0

448 (95%)

64

AAY21009

Zona pellucida protein Ba (Sparus aurata)

5E-164

333 (68%)

54

NP_956752

Eukaryotic translation elongation factor 2, like (Danio rerio)

0

738 (86%)

52

ABN80443

Creatine kinase 1 (Poecilia reticulata)

0

359 (94%)

47

P61155

40S ribosomal protein S19 (Pagrus major)

8E-64

121 (95%)

46

NP_571231

Keratin 5 (Danio rerio)

2E-138

270 (75%)

44

P84335

Tropomyosin (Pennahia argentata)

6E-139

279 (98%)

44

AAO43607

Serum lectin isoform 3 precursor (Salmo salar)

7E-17

45 (34%)

39

CAA63709

Chorion protein (Sparus aurata)

2E-149

283 (63%)

37

ABB17040

Heat shock cognate 70 (Fundulus heteroclitus)

3E-100

198 (96%)

32

BAA95131

Myosin light chain 2 (Pennahia argentata)

2E-87

162 (98%)

30

  1. aMost significant BLASTX hit is reported; bExtent of BLASTX hit aligned region (in amino acids), and percent identity over the aligned region.