Figure 6
From: Computational prediction and molecular confirmation of Helitron transposons in the maize genome
Silico 3 PCR products. Lanes: 1, size markers; 2, blank; 3, 4Co63; 4, A188; 5, A636; 6, B73; 7, BSSS53; 8, McC; 9, CML139; 10, H99; 11, I137TN; 12, Ki3; 13, M14; 14, Mo17; 15, W22; 16, W23; 17, bz-R. Silico 3 is predicted from B73 via our HelitronFinder software and is underlined in order to differentiate it from other lines. Both B73 and A636 show Helitron occupied sites, whereas lines McC, A188, W22, W23, and bz-R show Helitron vacant sites. In addition to the Helitron band amplified from B73, there was a faint band of the same size as the vacant site. We sequenced this product and confirmed it to be a vacant site.