Table 1 Summary of node, notochord, and DE genes with reduced expression in Foxa2 mutants.
Expression Group | Gene Symbol | Microarray* (MGU74v2A) | Microarray* (MOE430v2) | Q-PCR* | Reduced expression in Foxa2 mutants by whole mount in situ hybridization |
|---|---|---|---|---|---|
Known node and/or notochord markers | Foxd4 | -2.80 | -3.74 | -4.64 | Compare Figures 2A and 2D (n = 6/6) Ref: [34] |
| Â | Foxa1 | -1.18 | -1.56 | -1.69 | Ref: [10] |
| Â | T | -0.90 | -0.78 | -1.43 | |
| Â | Foxa2 | -1.28 | -0.87 | -- | -- |
| Â | Shh | -- | -1.43 | -- | |
| Â | Car3 | -- | -1.55 | -- | -- |
| Â | Cthrc1 | -- | -1.45 | -- | -- |
| Â | Chrd | -- | -1.49 | -- | -- |
| Â | Tmprss2 | -- | -0.36 | -- | -- |
| Â | Dynlrb2 | -- | -0.69 | -- | -- |
Novel node and/or notochord markers | Gal | -0.93 | -1.58 | -3.32 | |
| Â | Prnp | -0.65 | -0.76 | -0.30 | Data not shown (n = 3/3) |
| Â | Pim1 | -0.63 | -1.68 | -- | -- |
| Â | Smoc1 | -0.20 | -0.68 | -- | -- |
| Â | Gstm5 | -0.48 | -- | -- | -- |
| Â | Cyb561 | -0.40 | -- | -- | -- |
| Â | 1700027A23Rik | -- | -1.01 | -- | -- |
| Â | Josd2 | -- | -1.04 | -- | -- |
| Â | Mlf1 | -- | -0.87 | -- | -- |
| Â | 1700009P17Rik | -- | -1.26 | -- | -- |
Known DE markers | Sox17 | -0.83 | -0.87 | -0.58 | -- |
| Â | Cer1 | -1.40 | -1.87 | -2.32 | Ref: [38] |
| Â | Foxa2 | -1.28 | -0.87 | -- | -- |
| Â | Foxa1 | -1.18 | -1.56 | -1.69 | Ref: [10] |
| Â | Trh | -0.65 | -0.79 | -- | -- |
| Â | Shh | -- | -1.43 | -- | |
| Â | Gprc5c | -- | -1.64 | -- | -- |
| Â | Cyp26a1 | -- | -0.40 | -- | -- |
| Â | Tmprss2 | -- | -0.36 | -- | -- |
| Â | Cpn1 | -- | -0.79 | -- | -- |
Novel DE markers | Cldn4 | -0.60 | -- | -0.40 | Ref: (Burtscher & Lickert, 2008, manuscript submitted) |
| Â | Itga3 | -0.58 | -- | -0.60 | |
| Â | Cpm | -- | -2.45 | -- | -- |
| Â | Efhd2 | -- | -0.64 | -- | -- |
| Â | Ppp1r14a | -- | -0.90 | -- | -- |