Figure 1

Macro- and microscopic analysis of the fasting liver. A) Change in whole-body and liver weight during fasting as percentage of fed weight (n ≥ 8). Asterisks label significant changes (P < 0.01). The blue and pink lines represent the daily percentual change in body and liver weight, respectively, with the percent weight loss per day shown on the secondary y-axis. B) Histology of fed and 72 hour-starved livers (upper and lower panel, respectively). The sections were stained with hematoxylin and azophloxin (HA), and for the presence of glutamine synthetase (GS; pericentral expression) and carbamoylphosphate synthetase (CPS; periportal expression). The figures show that lobular architecture and metabolic zonation are unaffected by fasting. Bars: 0.1 mm.