Insulin-like growth factor-1 coordinately induces the expression of fatty acid and cholesterol biosynthetic genes in murine C2C12 myoblasts

Table 4 Cholesterol biosynthetic pathway genes up-regulated with IGF-1 treatment

Affy. Id.	Symbol	Genes up-regulated at 4 hrs	Fold Change
1425326_at	Acly	ATP Citrate Lyase *	2.7
1422478_a_at	Acas1	Acetyl CoA Synthetase *	3.3
1451271_a_at	Acat	Acetyl CoA Acetyltransferase	N/C
1423797_at	Acas	Acetyl CoA Acety Synthetase	3.1
1433443_at	Hmgcs1	HMG CoA Synthase 1	3.1
1427229_at	Hmgcr	HMGCoA Reductase	3.1
1430619_a_at	Mvk	Mevalonate Kinase	3.9
1427893_a_at	Pmek	Phosphomevalonate Kinase	3.0
1417303_at	Mvd	Mevalonate (diphospho) Decarboxylase	4.5
1451122_at	Idi1	Isopentenyl diphosphate delta isomerase	2.9
1423418_at	Fdps	Farnesyl diphosphate Synthetase	1.8
1415993_at	Sqe	Squalene epoxidase	2.1
1426913_at	Lss	Lanosterol Synthase	4.6
1422533_at	Cyp51	Lanosterol 14a demethylase (CYP51)	3.5
1423078_a_at	Sc4mo	Sterol C4 Methyl Oxidase	3.5
1416222_at	Nsdhl	NAD(P)H Steroid Dehydrogenase-like	2.4
1417871_at	Hsd17b7	17b Hydroxysteroid Dehydrogenase 7	6.1
1424709_at	Sc5d	Sterol C5 Desaturase	3.0
1448619_at	7Dhcr	7 dehydrocholesterol Reductase	3.2
		Uptake, breakdown & transport genes
1421821_at	Ldlr	LDL Receptor (Uptake)	2.0
1449227_at	M25oh	Cholesterol 25 hydroxylase (breakdown)	4.0
1429240_at	Startd4	Star-related Lipid transfer domain containing 4 (Transport)	2.8
		Regulatory genes
1426690_a_at	Srebp1a	Sterol Regulatory Element Binding Protein-1	1.1
1426744_at	Srebp2	Sterol Regulatory Element Binding Protein-2	1.4
1433520_at	Scap	SREBP Cleaveage Activator Protein	N/C
1448240_at	S1p	Site 1 protease	N/C
1417980_a_at	Insig2	Insulin Signal 2 (insulin induced)	1.7

The genes involved in cholesterol biosynthesis that are induced by about two-fold or greater levels at 4 hours are listed. All genes involved in cholesterol biosynthesis as well as those involved in cholesterol uptake (LDL receptor), break down (cholesterol 25 hydroxylase (M25oh)) and transport (Star-related Lipid transfer domain containing 4(Startd4)), are also up-regulated with IGF-1 treatment. Of the genes involved in the regulation of cholesterol biosynthesis only Srebp2 and Insig2 are feebly up-regulated, whereas Scap and S1p genes are unaffected. There is no available chip data on S2p. Genes common to the fatty acid and cholesterol biosynthetic pathways are indicated (*) and genes showing 'No Change' in expression levels are also listed (N/C).

ISSN: 1471-2164