Figure 2

Summary of microarray results. (A) Graphical representation of the six expression clusters: yellow corresponds to upregulation and blue to downregulation. Each row corresponds to an EST and each column to a developmental stage as labelled in Figure 1. Clusters I-VI consist of genes with their highest expression in the prawnchip, presettlement, presettlement and post-settlement, post-settlement, adult, and post-gastrulation stages, as diagrammed in (B). Presettlement orientation is oral to the left, postsettlement orientation is oral pointing out of the plane of the page. (C) Pie charts classifying the genes in each cluster into unique genes (blue-unique to Acropora), core genes (purple-matching a database entry in non-Metazoa, Radiata and Bilateria) and other (light yellow- any combination of any two of non-Metazoa, Radiata or Bilateria). Note that whilst 1084 unigenes were differentially expressed, the total number of unigenes in clusters is 1161. This is because 70 unigenes fall into two or more clusters, possibly due to the existence of splice variants for some unigenes.