Figure 5

Whole mount in situ hybridization of two carbonic anhydrases and two genes of unknown function which may be involved in calcification. Localisation of transcripts (dark purple) of carbonic anhydrases (A) C007-E7 and (B) A030-E11 and genes of unknown function (C) C012-D9 and (D) B036-D5 are shown in (1) metamorphosing larvae and (2-4) postsettlement polyps of three different ages (2 is youngest, 4 is oldest). C007-E7, C012-D9 and B036-D5 have expression patterns consistent with involvement in early calcification as they are expressed in the aboral end of metamorphosing larvae (A1, C1, D1) and the basal disc of early postsettlement polyps (A2, C2, D2), whilst A020-E11 (B) has an expression pattern consistent with a role in later calcification, as it is expressed in the septa (B4) of the oldest polyp, where the formation of calcified adult structures occurs.