Differentiation of similarly-sized products by qPCR. Dissociation curve (A) and gel image (B) of IGF1R in buffalo (BBU), hamster (A23), no-template control (NTC) and selected RH clones. River buffalo and hamster products are indistinguishable on agarose gels, but are easily separated by differential dissociation curve analysis. Note that in clones positive for buffalo DNA (e.g. #8) hamster DNA is also amplified, as expected.