Figure 4

Sensitivity of qPCR method. Dissociation curve (A) and gel image (B) of RM151 in buffalo (BBU), hamster (A23) and selected RH clones. The left peak on the dissociation curve is presumed to be primer-dimer (~40 bp, as seen in the gel image), while the right peak is the target PCR product (barely visible or not at all in the gel image). This marker could not be scored by a gel-based method, but clones positive for buffalo DNA (#8, #22) are easily identified after dissociation curve analysis.