Figure 5From: Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B. bubalis) chromosome 20Ambiguous qPCR typing of AKT2. Dissociation curve (A) and gel image (B) of AKT2 in buffalo (BBU), hamster (A23) and selected RH clones. Most "positive" clones (~90 bp target band present on gel) produced dissociation curves similar to the buffalo control (BBU, #94, #98) and most "negative" clones (target band absent on gel) produced dissociation curves similar to the hamster control (A23, #102); however, a number of clones produced intermediate or shifted dissociation curves that could not be scored convincingly (e.g. #104). Extraneous products and small target product size may have contributed to the variable dissociation curves for this marker.Back to article page