Ambiguous qPCR typing of AKT2. Dissociation curve (A) and gel image (B) of AKT2 in buffalo (BBU), hamster (A23) and selected RH clones. Most "positive" clones (~90 bp target band present on gel) produced dissociation curves similar to the buffalo control (BBU, #94, #98) and most "negative" clones (target band absent on gel) produced dissociation curves similar to the hamster control (A23, #102); however, a number of clones produced intermediate or shifted dissociation curves that could not be scored convincingly (e.g. #104). Extraneous products and small target product size may have contributed to the variable dissociation curves for this marker.