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Table 3 Prediction of signal peptides of proteins identified in the culture supernatant

From: Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae

Protein

Signal peptide probability

Maximum cleavage site probability

Predicted signal length (aa)

Predicted signal peptide

Predicted secretion system

Predicted function

RL0196

1.000

0.999

20

MKKSLLTLFAVAAMSTTALA

GEP

basic membrane lipoprotein

RL0489

1.000

1.000

23

MKKSVLAFGALALGVTFSAPVMA

GEP

sugar-binding protein

RL0518

1.000

1.000

32

MSIVKSLLSRR AFTALAGAAVIATAMPAPSFA

TAT

ribose-binding protein

RL0778

1.000

0.956

23

MKKISVLLAATALISVMATSAWS

GEP

dipeptide-binding protein

RL1369

0.999

0.803

25

MAMHGSSLGLLALVLLAFPPVAARA

GEP

pentapeptide repeat protein

RL2375

1.000

0.944

24

MKKLALALAATAALVLSIGSAAEA

GEP

basic membrane lipoprotein

RL2404

1.000

0.983

23

MKLFYLAFAGVLYLASFAGDAFA

GEP

peptidyl prolyl cis-trans isomerase

RL2697

0.999

0.933

28

MMNIAGLGRLAAATVVLSGLAFGSAVKA

GEP

COG3184

RL2753

1.000

1.000

25

MLNSTRIFAAASIAAMSLFAGSAMA

GEP

arginine/ornithine-binding protein

RL3329

1.000

0.975

25

MHRSLASCSALALLFALALAGGAAA

GEP

membrane bound lytic tranglycosylase

RL3745

1.000

0.999

23

MKKSLLSAVALTAMVAFSGNAWA

GEP

Leu/Ile/Val-binding protein (BraC)

RL4218

1.000

0.998

22

MTLRTFLLGACSALAFAGMASA

GEP

sorbitol-binding protein

RL4651

1.000

1.000

29

MTKLNRNFRMLSAGAALSLLMMAAPSAFA

GEP

dipeptide-binding protein

pRL90140

0.000

0.000

-

-

PrsDE

parallel beta-helix repeat protein

pRL100309

0.000

0.000

-

-

PrsDE

cadherin-like protein

pRL100451

0.000

0.000

-

-

PrsDE

RapA2

RL1580

0.000

0.000

-

-

PrsDE

nucleoside diphosphate kinase

RL2412

0.000

0.000

-

-

PrsDE

glycosyl hydrolase

RL2961

0.000

0.000

-

-

PrsDE

cadherin-like protein

RL3024

0.000

0.000

-

-

PrsDE

PlyB

RL0718

0.002

0.001

-

-

Flagellum

flagellin

RL0720

0.012

0.005

-

-

Flagellum

flagellin

RL0728

0.032

0.029

-

-

Flagellum

flagellar hook

  1. Signal peptide prediction was done using the hidden Markov model of the SignalP 3.0 software [66]. Probabilities for the presence of a signal peptide and for the correct identification of the cleavage site are given. The TAT signal prediction software TATP 1.0 predicts the same signal peptide length for the predicted TAT substrate RL0518. The twin-arginine motif of the RL0518 protein is in bold. The prediction of the secretion system involved in the export of the identified proteins was based on signal peptide prediction (GEP and TAT), observed absence from the culture supernatant of the prsD mutant (PrsDE) and similarity to known proteins (Flagellum).