Figure 11

Analysis of Boophilin-like peptidase inhibitors. Multiple sequence alignment (CLUSTALX) of protein sequences identified in a cDNA library of unfed/2 d fed or 6 d fed Dermacentor variabilis midguts (DvM) and thrombin in hibitor-like proteins found on genbank. Black shading indicates identity and grey shading indicates similarity. Boxed area indicates the amino acids that constitute the peptidase recognition loop. * P1 site, the primary recognition residue. Arrowhead indicates the start of the second Kunitz domain. Shading represents 100% identity (black) or similarity (grey) among the sequences. Alignments were conducted using CLUSTALX. D. variabilis (Dv), Homo sapiens alpha-1-microglobulin bikunin (Hs), Pancreatic trypsin inhibitor precursor (BPTI), Amblyomma hebraeum (Ah), Rhipicephalus (Boophilus) microplus (Bm), Ixodes scapularis (Is), Ornithodoros moubata (Om), O. savignyi (Os).