Analysis of mucins. (A) Phylogenetic tree based on maximum likelihood analysis of a Dermacentor variabilis midgut protein and published mucin-like protein sequences. The transcript identified in this analysis is in bold (DvM). Phylogenetic analysis was conducted on protein alignments using Tree Puzzle version 5.2. Values at nodes represent calculated internal branch node support (1000 replications). (B) Multiple sequence alignment (CLUSTALX) of protein sequences identified in a cDNA library of unfed/2 d fed or 6 d fed D. variabilis midguts (DvM) and published sequences found on Genbank. The conserved cysteines are highlighted. Shading represents 100% identity (black) or similarity (grey) among the sequences. Alignments were conducted using CLUSTALX. D. variabilis (Dv), Ixodes scapularis (Is), Haemaphysalis longicornis (Hl), Ornithodoros moubata (Om), Argas monolakensis (Am), Lutzomyia longipalpis (Ll), Phlebotomus papatasi (Pp).