Analysis of microsomal GST protein sequences. (A) Phylogenetic tree based on maximum likelihood analysis Dermacentor variabilis midgut protein and published microsomal GST sequences. The transcript identified in this analysis is in bold (DvM). Phylogenetic analysis was conducted on protein alignments using Tree Puzzle version 5.2. Values at nodes represent calculated internal branch node support (1000 replications). (B) Multiple sequence alignment (CLUSTALX) of protein sequences identified in a cDNA library of unfed/2 d fed or 6 d fed D. variabilis midguts (DvM) and published microsomal GST 3 sequences found on genbank. Shading represents 100% identity (black) or similarity (grey) among the sequences. Alignments were conducted using CLUSTALX. D. variabilis (Dv), Rattus norvegicus (Nr), Mus musculus (Mm), Canis familiaris (Cf), Xenopus tropicalis (Xt), Strongylocentrotus purpuratus (Sp), Escherichia coli (Ec), Yersinia pestis (Yp), Homo sapiens (Hs), Bordetella pertussis (Bp), Prochlorococcus marinus (Pm), Drosophila melanogaster (Dm), Anopheles gambiae (Ag).