Differential replication dynamics for large and small Vibrio chromosomes affect gene dosage, expression and location

Table 2 Doubling times, replication times and theoretical origin/terminus ratios for V. parahaemolyticus, V. cholerae and V. vulnificus

Species	Growth condition	Doubling time^§	Replication time large chr*	Replication time small chr*	Ori/ter ratio large chr^#	Ori/ter ratio small chr^#
V. para	3%LB37°C	12–14 min	27.4 min	15.6 min	4.31	2.30
V. para	3%M937°C	50–60 min	27.4 min	15.6 min	1.41	1.22
V. para	3%LB20°C	36–42 min	27.4 min	15.6 min	-	-
V. chol	LB37°C	16–20 min	23.8 min	10.0 min	2.50	1.47
V. vuln	3%LB37°C	18–22 min	28.3 min	14.6 min	2.67	1.66

^§ Doubling times were determined for OD₆₀₀ values around 0.5.
*Replication times were estimated considering a bidirectional replication speed of 1000 nt/s for both chromosomes of V. parahaemolyticus, V. cholerae and V. vulnificus
^#Ratios were calculated using the average values for the estimated doubling times.
LB = Luria-Bertani medium containing 1% NaCl, 3%LB = Luria-Bertani medium containing 3% NaCl, 3%M9 = M9 medium containing 3% NaCl and 0.4% glucose.

ISSN: 1471-2164