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Table 2 Doubling times, replication times and theoretical origin/terminus ratios for V. parahaemolyticus, V. cholerae and V. vulnificus

From: Differential replication dynamics for large and small Vibrio chromosomes affect gene dosage, expression and location

Species Growth condition Doubling time§ Replication time large chr* Replication time small chr* Ori/ter ratio large chr# Ori/ter ratio small chr#
V. para 3%LB37°C 12–14 min 27.4 min 15.6 min 4.31 2.30
V. para 3%M937°C 50–60 min 27.4 min 15.6 min 1.41 1.22
V. para 3%LB20°C 36–42 min 27.4 min 15.6 min - -
V. chol LB37°C 16–20 min 23.8 min 10.0 min 2.50 1.47
V. vuln 3%LB37°C 18–22 min 28.3 min 14.6 min 2.67 1.66
  1. § Doubling times were determined for OD600 values around 0.5.
  2. *Replication times were estimated considering a bidirectional replication speed of 1000 nt/s for both chromosomes of V. parahaemolyticus, V. cholerae and V. vulnificus
  3. #Ratios were calculated using the average values for the estimated doubling times.
  4. LB = Luria-Bertani medium containing 1% NaCl, 3%LB = Luria-Bertani medium containing 3% NaCl, 3%M9 = M9 medium containing 3% NaCl and 0.4% glucose.