Figure 1
Growth and antibiotic synthesis kinetics of M145 and YSK4425. (a) Growth measured using optical density at 450 nm. (b) and (c) Spectrophotometric measurements of antibiotic titers for undecylprodigiosin and actinorhodin. Time profiles for M145 (â—‹, solid blue line) and YSK4425 (â–³, dashed red line) are shown. The curves represent data from one of two reproducible experiments. (d) Photograph shows the dramatic difference in antibiotic synthesis between the M145 and YSK4425. Pictures are culture samples taken ~4 days after inoculation, indicating that the observed absence of actinorhodin synthesis was a genuine abolishment rather than a delay in synthesis.