Skip to main content

Table 4 Primers used for MLST

From: Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains

Locus Function Forward primer Reverse primer Location (a)
dinB DNA polymerase 5'-GTT TTC CCA GTC ACG ACG TTG TAT GAG AGG TGA GCA ATG CGT A-3' 5'-TTG TGA GCG GAT AAC AAT TTC CGT AGC CCC ATC GCT TCC AG-3' 282,284 – 282,734
icdA Isocitrate dehydrogenase 5'-GTT TTC CCA GTC ACG ACG TTG TAA TTC GCT TCC CGG AAC ATT G-3' 5'-TTG TGA GCG GAT AAC AAT TTC ATG ATC GCG TCA CCA AAY TC-3' 1,118,658 – 1,187,173
pabB p-aminobenzoate synthase 5'-GTT TTC CCA GTC ACG ACG TTG TAA ATC CAA TAT GAC CCG CGA G-3' 5'-TTG TGA GCG GAT AAC AAT TTC GGT TCC AGT TCG TCG ATA AT-3' 1,807,273 – 1,807,740
polB Polymerase PolII 5'-GTT TTC CCA GTC ACG ACG TTG TAG GCG GCT ATG TGA TGG ATT C-3' 5'-TTG TGA GCG GAT AAC AAT TTC GGT TGG CAT CAG AAA ACG GC-3' 65,220 – 64, 773
putP Proline permease 5'-GTT TTC CCA GTC ACG ACG TTG TAC TGT TTA ACC CGT GGA TTG C-3' 5'-TTG TGA GCG GAT AAC AAT TTC GCA TCG GCC TCG GCA AAG CG-3' 1,074,708 – 1,075,163
trpA Tryptophan synthase subunit A 5'-GTT TTC CCA GTC ACG ACG TTG TAG CTA CGA ATC TCT GTT TGC C-3' 5'-TTG TGA GCG GAT AAC AAT TTC GCT TTC ATC GGT TGT ACA AA-3' 1,338,658 – 1,338,098
trpB Tryptophan synthase subunit B 5'-GTT TTC CCA GTC ACG ACG TTG TAC ACT ATA TGC TGG GCA CCG C-3' 5'-TTG TGA GCG GAT AAC AAT TTC CCT CGT GCT TTC AAA ATA TC-3' 1,339,430 – 1,338,837
uidA Beta-glucuronidase 5'-GTT TTC CCA GTC ACG ACG TTG TAC ATT ACG GCA AAG TGT GGG TCA AT-3' 5'-TTG TGA GCG GAT AAC AAT TTC CCA TCA GCA CGT TAT CGA ATC CTT-3' 1,615,010 – 1,614,411
OF Sequencing primer (b) 5'-GTT TTC CCA GTC ACG ACG TTG T-3'   
OR Sequencing primer (b) 5'-TTG TGA GCG GAT AAC AAT TTC-3'   
  1. (a) Based on Escherichia coli strain 536, complete genome, GenBank accession CP000247.
  2. (b) PCR primers have the corresponding universal sequence in 5'.