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Table 1 Characteristics of cDNA libraries and EST data.

From: Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure

Cell treatment

Abbreviation

Vector(s)

Library prefix(es)

Total valid EST reads

5' reads

3' reads

No. of predicted genes hit

Vegetative growth, rich medium

RCH

pcDNA3.1

TTB, TTC, TTD

10229

5270

4959

661

Vegetative growth, rich medium, plus Cu/Cd

HVM

pBluescriptIISK+

TTS

3160

3160

0

712

Vegetative growth, rich medium, plus TSA

TSA

pDNR-LIB

FTS

2651

2651

0

1810

Vegetative growth, minimal medium

MIN

pDNR-LIB

FMM

1809

1809

0

1157

Starvation

STV

pBluescriptIISK+

TT1

23321

12820

10501

4647

Conjugation

CNJ

pBluescriptIISK+, pDNR-LIB

TTE, FCO

18837

17813

1024

6568

Total

   

60007

43523

16484

9709