Table 3 Genes used for microarray validation with qRT-PCR
From: Genome-wide transcriptional response of an avian pathogenic Escherichia coli (APEC) pst mutant
No. | Gene | Gene title | Microarray Fold change (Log2) | qRT-PCR Fold change (Log2) |
|---|---|---|---|---|
1 | phoA | Alkaline phosphatase | 6.816 | 7.600 |
2 | gadW | DNA-binding transcriptional activator | 2.773 | 2.303 |
3 | cdh | CDP-diacylglycerol phosphotidylhydrolase | 2.099 | 1.607 |
4 | ycgV | Predicted adhesin | 2.022 | 2.483 |
5 | yddV | Diguanylate cyclase | 0.851 | 0.890 |
6 | ydeQ | Predicted fimbrial-like adhesin | -0.651 | -0.573 |
7 | rpoD | RNA polymerase sigma factor | -0.640 | -1.087 |
8 | crl | Curlin genes transcriptional activatory protein | -1.331 | -1.037 |
9 | thiF | Thiamine biosynthesis protein | -2.152 | -1.787 |
10 | yhcN | Hypothetical protein | -2.967 | -5.680 |
11 | lexA | Regulator for SOS regulon | -1.198 | -2.377 |
12 | oxyS | Oxidative stress regulator | -0.864 | -1.777 |
13 | hfq | RNA-binding protein | -0.638 | -1.340 |
14 | yidB | Hypothetical protein | -0.10 | -0.013 |
15 | yeiR | Predicted enzyme | -0.04 | 0.212 |