Figure 3From: Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feedingValidation of sequence quality of FLcDNAs. Sequence accuracy was measured as the percentage of the 4,664 FLcDNAs which, with 100%, 95.0–99.9%, 90.0–94.9% or < 90.0% of their sequence length, exceeded Phred30, Phred40, Phred50 or Phred60 sequence quality thresholds. All 4,664 FLcDNAs exceeded the Phred30 quality thresholds (calculated as less than 1 error in 103 sequenced nucleotides) over 100% of their sequence length. Even at the threshold level of Phred60 (calculated as less than 1 error in 106 sequenced nucleotides) the majority (61.2%) of the FLcDNA sequences met this very high sequence quality score over > 90.0% of their length.Back to article page