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Figure 3 | BMC Genomics

Figure 3

From: Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feeding

Figure 3

Validation of sequence quality of FLcDNAs. Sequence accuracy was measured as the percentage of the 4,664 FLcDNAs which, with 100%, 95.0–99.9%, 90.0–94.9% or < 90.0% of their sequence length, exceeded Phred30, Phred40, Phred50 or Phred60 sequence quality thresholds. All 4,664 FLcDNAs exceeded the Phred30 quality thresholds (calculated as less than 1 error in 103 sequenced nucleotides) over 100% of their sequence length. Even at the threshold level of Phred60 (calculated as less than 1 error in 106 sequenced nucleotides) the majority (61.2%) of the FLcDNA sequences met this very high sequence quality score over > 90.0% of their length.

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