Figure 5

Mapping FLcDNAs to the poplar genome. 4,664 poplar FLcDNAs were aligned to the genome using BLAT with default parameters (match length ≥ 11 bp, BLAT score ≥ 30, sequence identity ≥ 90%). Prior to alignment, the 5' second strand primer adaptor sequences (SSPA) and polyA tails were removed. Among 4,642 poplar FLcDNAs that exceeded the minimal criteria for a match to the genome, 3,847 mapped to chromosomes whereas the remainder mapped to scaffold segments. Colored bars indicate the cDNA library of origin for those FLcDNAs mapping to one of the 19 poplar chromosomes. Applying a higher stringency threshold (sequence identity ≥ 95%, alignment coverage ≥ 95%), 4,487 or 96.2% of poplar FLcDNAs could be mapped to the genome.