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Table 2 The list of transcripts associated with cuticle hardening that are up-regulated in intermolt (Cy5) crabs when compared against crabs in the post-molt stage (Cy3).

From: Differential expression profiling of components associated with exoskeletal hardening in crustaceans

Clone IDs

(Accession #)

BLAST results

Score (Bits)

E value

M *

t *

P-value*

Adjusted

P-value*

PpHEM7

(EF110537)

AY861677 Cancer magister hemocyanin AAW57890 hemocyanin C. magister

290

455

6e-75

1e-126

-5.147

-4.716

-4.432

-10.466

-17.112

-14.02

0

0

0

0.004

0.002

0.002

PpHEM3

(EF110533)

AF249297 Callinectes sapidus hemocyanin subunit mRNA

AAW57891 hemocyanin subunit 3 C. magister

807

432

0.0

1e-119

-4.849

-3.951

-16.719

-14.337

0

0

0.002

0.002

PpTRY1

(EF120993)

AB205408 Seriola quinqueradiata mRNA for trypsinogen

CAA75310 trypsin Litopenaeus vannamei

75.8

318

2e-10

2e-85

-4.655

-4.434

-4.193

-4.154

-9.19

-13.924

-11.15

-12.959

0

0

0

0

0.007

0.002

0.004

0.003

PpHEM1

(EF110531)

AY861676 C. magister hemocyanin subunit 1

AAW57889 hemocyanin subunit 1 C. magister

103

335

9e-19

1e-90

-4.473

-4.393

-4.112

-9.907

-15.061

-15.684

0

0

0

0.005

0.002

0.002

PpTRY2

(EF120994)

AAL67442 trypsin Paralithodes camtschaticus

62.0

1e-08

-4.473

-10.021

0

0.005

PpHEM4

(EF110534)

AF249297 C. sapidus hemocyanin subunit mRNA

AAF64305 hemocyanin subunit C. sapidus

870

472

0.0

6e-132

-4.402

-4.295

-4.027

-3.954

-3.784

-3.75

-3.707

-2.331

-14.753

-14.847

-15.206

-14.946

-14.715

-14.898

-12.568

-6.86

0

0

0

0

0

0

0

0.001

0.002

0.002

0.002

0.002

0.002

0.002

0.003

0.018

PpHEM2

(EF110532)

AY861677 C. magister hemocyanin subunit 2

AAW57890 hemocyanin subunit 2 C. magister

176

160

8e-41

1e-47

-4.273

-9.162

0

0.007

PpHEM5

(EF110535)

AF249297 C. sapidus hemocyanin subunit

AAW57893 hemocyanin subunit 5 C. magister

327

195

3e-86

2e-89

-4.18

-12.698

0

0.003

PpHEM6

(EF110536)

AF249297 C. sapidus hemocyanin subunit mRNA

AAW57891 hemocyanin subunit 3 C. magister

807

432

0.0

1e-119

-4.155

-4.083

-3.999

-3.977

-3.946

-3.869

-3.518

-16.298

-11.88

-15.838

-14.718

-15.306

-14.029

-13.331

0

0

0

0

0

0

0

0.002

0.003

0.002

0.002

0.002

0.002

0.003

PpTRY3

(EF120995)

Y15041 PVTRYPSN3 Penaeus vannamei trypsin gene

CAA75311 trypsin Litopenaeus vannamei

63.9

253

6e-07

3e-66

-4.119

-4.017

-3.972

-3.246

-3.225

-10.005

-11.911

-12.269

-8.569

-10.838

0

0

0

0

0

0.005

0.003

0.003

0.008

0.004

PpHEM8

(EF110538)

AF249297 C. sapidus hemocyanin subunit

AAF64305 hemocyanin subunit C. sapidus

95.6

109

3e-16

3e-22

-3.41

-11.878

0

0.003

PpCTRY1

(EF120996)

CAA71673 Chymotrypsin BII L. vannamei

32.7

4.9

-2.935

-9.127

0

0.007

EF120998

AAD16454 clotting protein precursor Pacifastacus leniusculus (crayfish)

38.9

0.10

-2.83

-8.196

0

0.01

PpCTRY2

(EF120997)

HAY12273 H. armigera (cotton bollworm – arthropod) mRNA for putative serine protease (chymotrypsin-like protease)

CAA71672 Chymotrypsin BI Litopenaeus vannamei

50.1

159

0.008

6e-38

-2.433

-2.021

-8.984

-6.564

0

0.001

0.007

0.021

EF120999

CAD20734 carcinin Carcinus maenas (antibacterial protein)

71.2

2e-11

-1.696

-5.514

0.002

0.036

  1. M = log2 fold change in expression, t = t-statistic, and the P value = t-distribution. The adjusted P values were calculated using the False Discovery Rate (FDR) procedure. Negative values of M and t indicate up-regulation in the Cy5 sample (* multiple values represent several cDNAs all forming one contig).