Use of pyrosequencing and DNA barcodes to monitor variations in Firmicutes and Bacteroidetescommunities in the gut microbiota of obese humans

Table 2 Specificity of the Firmicutes and Bacteroidetes DNA barcodes

			% of Hit with *Bacteroidetes*barcode		% of Hit with *Firmicutes*barcode
Phylum	Number of sequences in RDP-II	Number of sequences in Greengenes	RDP-II	Greengenes	RDP-II	Greengenes
Bacteroidetes	18272	4718	_	_	0.41	0
Firmicutes	45757	16023	0.04	0.057	_	_
Proteobacteria	41277	38449	0	0.049	0.18	0.27
Actinobacteria	11366	12736	0	0.0079	1.28	1.07
Cyanobacteria	2164	1873	0.05	0.053	32.76	29.31
Spirochaetes	1547	1677	0	0.059	0	0.18
Verrucomicrobia	953	324	0	0	0	0
Planctomycetes	864	553	0.23	0.54	0	0.18
Chloroflexi	713	643	0	0	0	0.77
Acidobacteria	782	865	0	0	0	0
Aquificae	739	1131	0	0	0	0
Fusobacteria	421	198	0	0	97.38	90.40
Nitrospirae	376	156	0	0	0	0
Deinococcus-Thermus	381	419	0	0.23	0	0.24
Chlamydiae	178	167	0	0	0	0
Deferribacteres	71	64	0	0	0	0

RDP-II: Ribosomal Database Project II. For each bacterial phylum of the RDP-II and Greengenes databases, the percentage of sequences possessing the Bacteroidetes and/or the Firmicutes DNA barcode was calculated.

ISSN: 1471-2164