Figure 4
From: A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans
A new reporter construct allowing in vivo analysis of gene expression in C. albicans. A) Gene replacement by beta-galactosidase following 1- wild-type or mutant promoter cloning, 2- PCR amplification of the promoter-lacZ fusion and 3- in vivo gene replacement by transformation and homologous recombination. B) PCR confirmation of our in vivo inserted beta-galactosidase constructs. C) Beta-galactosidase activity of the promoters of the OPI3, LYS9 and RPL11 genes. YFG stands for Your Favorite Gene.