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Table 1 Comparison of fold-change values from qRT-PCR and microarray for selected target genes.

From: Development and validation of a gene expression oligo microarray for the gilthead sea bream (Sparus aurata)

TARGET TRANSCRIPT SAPD ID Fold Changea
   Real-time RT-PCR Microarray Probe_1 Microarray Probe_2
Apolipoprotein E1 SAPD02358 0.00362 0.12263 0.1276
Flap endonuclease 1 SAPD04884 0.07811 0.16678 0.18717
Ovostatin SAPD01680 0.00525 0.13731 0.14149
Myosin SAPD10294 0.16533 0.25064 0.21589
Serotransferrin SAPD01126 0.02439 0.11883 0.10408
Glutamate R7 SAPD19202 3.58394 4.30343 4.61982
Methionine aminopeptidase 2 SAPD26496 12.9384 6.88419 6.91868
L-lactate dehydrogenase SAPD00597 3.09142 2.99927 3.00815
Serine racemase SAPD19150 40.9397 18.3846 19.9929
Retinal cone arrestin-3 SAPD02277 2232.6 29.4717 44.2798
Phosphoglycerate kinase 1 SAPD03464 1.84119 1.26289 1.12021
Malate dehydrogenase 1 SAPD02236   1.05 0.99
  1. a Fold change is calculated as ratio of Stage 4 vs Stage 1, using mean signal intensity across five biological replicates of each stage. Values below 1.0 indicate down-regulation in Stage 4.