Figure 2

Increased sensitivity of tae1 Δ to different translation inhibitory drugs. Deletion of TAE1 confers increased sensitivity to different drugs that target translation. Decreasing numbers of wild type and mutant (tae1Δ) yeast cells were spotted on solid media. The media was supplemented with sub-inhibitory concentrations of paromomycin (13 mg/ml), 3-AT (22 mg/ml), cycloheximide (45 ng/ml), or none (used as a control and shown in the top panel). Standard organic (YEPD) media was used for cycloheximide and synthetic complete (SC) media was used for paromomycin and 3-AT plates, and for the control plate shown here. Yeast cells were grown to mid-log phase and diluted 10-3 to 10-6 folds. Twenty microliters of each dilution (gradually decreasing) was spotted onto the media and grown at 30°C for 1–2 days. Deletion of TAE1 conferred increased sensitivity to paromomycin and 3-AT. Occasional sensitivity to cycloheximide was assumed to be an artifact.