Skip to main content

Advertisement

Figure 4 | BMC Genomics

Figure 4

From: Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis

Figure 4

Protein synthesis profile in the presence and absence of Tae1. In (A) total protein synthesis is measured using [35S] methionine incorporation. The average count for [35S] methionine incorporation for wild type is 11,356,073 (± 1,300,000) counts, which is set to 100%. In the absence of Tae1 protein, total protein production is reduced by approximately 22%. t-test analysis indicates that the observed difference is statistically significant with the p-value of 0.05. In (B) the efficiency of protein synthesis is measured using an inducible β-galactosidase reporter gene. The average β-galactosidase activity for wild type is 7.51 (± 0.6) units, which is set to 100%. In the absence of Tae1 protein, an approximately ten-fold reduction in β-galactosidase production is observed.

Back to article page