Table 2 ssDNA sequences of the oligonucleotides used in padlocks, target molecules and primers (5'-3' orientation).

Cotton sp.

CTGGGCTGAGAACAACATTCTGACTCACCTCAAACCA

AAGTGTGCCAGACGCTCGAA

CTTTAAATCTTTGGAGGG

[AJ132636] (sad1)

122

Maize sp.

CTGTGGCATCATCACTGGCATCGT

GTACTACATTCGTGCGATGG

TTAGGCGTCATCAT

[AF371266] (zein)

124

Canola sp.

GTGACGCATACGTTCTATAACATCAGCCTGTCC

CGTCGCGTTAGACAGCTCAT

CCGATCTTTCTTGTATTC

[DQ173668] (ACCase)

118

Soya sp.

GCATCATAGGTAATGAGAACCTTGGCTACTTTATTGTTGGCC

ACTCCAGTGCCAAGTACGAT

AGAGGCTGGTGGAG

[K00821] (lectin1)

142

RRS event

GATCCCAAATAGTTTTGTTTTTCTAACAACGAGAAGCTATATGTAGATGCTATT

AACAACGATGAGACCGGGCT

TCAAACAGTTCTTCTCC

[AJ308515]

138

MON810

GGCAATGGCAAAGGATGTTAAACGTTAGAGTCCTTCGT

TGCCCTATTGTTGCGTCGGA

AAAGTGACAGATAGCTG

[AF434709]

141

Bt176 event

GAACATCAGATCTCGGTGACGGGCAGGACC

TAATCTAATTCTGGTCGCGG

GCATCAATGGAGGAGA

[AJ878607]

113

CaMV P35S

CGAAGGATAGTGGGATTGTGCGTCATCCCTTACG

CCACGAGCTGTAATCCGGTA

ATAGAGGAAGGGTCTTG

[V00141]

118

FMV P35S

TCTTCGGTGGATGTCTTTTTCTGAAACTTACTGACCATGATG

GTGATTAAGTCTGCTTCGGC

GCCCACTAACTTTAAG

[X06166]

125

bar

TCGATGTAGTGGTTGACGATGGTGCAGACCG

CGAGTGCTCCGTGCGAAATA

TGACCGTGCTTGTC

[AY346130]

112

SpikeLock

CGTCGGACAGGTTACTTTCGAAGAGCCGGAATACTC

GCTGAGGTCGATGCTGAGGTCGCA (cZIP-P for Positioning)

CGAAGGTCATATCTCG

123

ZB3

CGTGCAAGTTACCGAGCTGA

20

primer sequence

Fwd primer

GCAAGAGATGGGCTACAGAGGAT

23

Fwd25 primer

CCGCAAGAGATGGGCTACAGAGGAT

25

Rev primer

GGACAGACACGCTAAGACAGAACT

24