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Table 1 Proteins identified by LC/MS/MS

From: Overcoming function annotation errors in the Gram-positive pathogen Streptococcus suis by a proteomics-driven approach

Gene locus, Annotated protein function

Predicted subcellular localization

Prediction algorithm

ssu05_0753, MRP

Cell wall

PSORTb v 2.0

ssu05_1982, Subtilisin-like serine protease

Cell wall

PSORTb v 2.0

ssu05_1968, DNA nuclease

Cell wall

PSORTb v 2.0

ssu05_0196, hypothetical protein SSU05_0196

Cell wall

PSORTb v 2.0

ssu05_1311, hypothetical protein SSU05_1311

Cell wall

PSORTb v 2.0

ssu05_0965, agglutinin receptor

Cell wall

PSORTb v 2.0

ssu05_2064, Type II secretory pathway, pullulanase PulA and related glycosidases

Cell wall

PSORTb v 2.0

ssu05_1371, Ribonucleases G and E

Cell wall

PSORTb v 2.0

ssu05_1295, hypothetical protein SSU05_1295

Cell wall

PSORTb v 2.0

ssu05_0214, ABC-type xylose transport system, periplasmic component

Cell wall

PSORTb v 2.0

ssu05_1663, Methyl-accepting chemotaxis protein

Cell wall

PSORTb v 2.0

ssu05_0473, Ribonucleases G and E

Cell wall

PSORTb v 2.0

ssu05_0700, ATPase (PilT family)

Lipoprotein

LipoP

ssu05_1083, Uncharacterized ABC-type transport system, periplasmic component/surface lipoprotein

Lipoprotein

LipoP

ssu05_2133, ABC transporter substrate-binding protein – maltose/maltodextrin

Lipoprotein

LipoP

ssu05_0513, Membrane-fusion protein

Membrane

TMHMM

ssu05_1022, hypothetical protein SSU05_1022

Membrane

TMHMM

ssu05_1635, Predicted xylanase/chitin deacetylase

Membrane

TMHMM

ssu05_1354, Cell division protein FtsI/penicillin-binding protein 2

Membrane

TMHMM

ssu05_1509, Negative regulator of septation ring formation

Membrane

TMHMM

ssu05_2173, LysM repeat protein

Membrane

TMHMM

ssu05_1579, Ammonia permease

Membrane

PSORTb v 2.0, TMHMM

ssu05_1292, Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily

Membrane

PSORTb v 2.0, TMHMM

ssu05_1380, ABC-type antimicrobial peptide transport system, permease component

Membrane

TMHMM

ssu05_1282, Predicted membrane protein

Membrane

PSORTb v 2.0, TMHMM

ssu05_0332, hypothetical protein SSU05_0332

Secreted

SignalP

ssu05_0811, Subtilisin-like serine protease

Secreted

SignalP

ssu05_1682, extracellular serine protease

Secreted

SignalP

  1. Surface proteins identified after protease treatment of cultured live cells of Streptococcus suis serotype 2, strain 235/02. The table reports: 1) the accession number of the genes encoding the identified proteins, 2) the predicted cellular localization of the proteins, 3) the algorithms used for such predictions.