Figure 5

qPCR analysis confirms that luminal ER^- cells are enriched for expression of LPS receptor pathway genes. Data from qPCR analysis of CD14, Irak2, Ly94, Tlr4 and Tnf gene expression in triplicate independent samples of basal/myoepithelial cells, luminal ER^- cells and luminal ER⁺ cells. Each data point is the mean level of expression, ± 95% confidence intervals, across the three samples of that population relative to the comparator sample. The 'round robin' comparison method and assignment of genes to the populations were carried out as described in the Methods.