Tissue specific patterns of expression for candidate spermatophore proteins assayed via reverse transcription PCR. Patterns of expression for the three groups of highly abundant accessory gland transcripts and α-tubulin were assayed in three males (abdomen and thorax) and three females (abdomens only). PCR primers were designed to amplify universally from all transcripts in each of the three groups, two corresponding to tyrosine-rich proteins and one to asparagine-rich proteins. First-strand cDNA synthesized from equal concentrations of total RNA was used as template. For each primer set, equal amounts of PCR amplicon (ranging between 4 and 9 μL) were electrophoresed on 1.2% agarose gels. NC = negative control (no template added to PCR mix).