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Table 1 Summary of EST, BLAST, and SignalP analyses from H. erato and H. melpomene male accessory gland cDNA libraries.

From: EST analysis of male accessory glands from Heliconius butterflies with divergent mating systems

  Heliconius erato Heliconius melpomene
EST results   
   Number of clones sequenced 1152 1148
   High Quality ESTs1 936 (81%) 1033 (89%)
   Number of unigenes 371 340
   Average sequence length (base pairs) 641 597
BLAST results 2   
   Unigenes with significant BLAST hits to GenBank protein or nucleotides3 (E-value < 10-5) 257 (69%) 235 (69%)
   Unigenes with significant BLASTX hits to GenBank proteins (E-value < 10-5) 216 (58%) 218 (64%)
   Unigenes with significant BLASTN hits to GenBank nucleotides (E-value < 10-5) 151 (40%) 150 (44%)
SignalP results 2   
   Unigenes with predicted signal peptides4 86 (24%) 92 (28%)
  1. 1ESTs > 100 bp after trimming raw sequences of vector sequence, low-scoring base calls, and poly-A tails. 2Percentages given in reference to total number of unigenes. 3Includes results from both BLASTN and BLASTX searches. 4We required a positive result from both hidden markov models and neural network methods implemented in SignalP.