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Table 1 Summary of EST, BLAST, and SignalP analyses from H. erato and H. melpomene male accessory gland cDNA libraries.

From: EST analysis of male accessory glands from Heliconius butterflies with divergent mating systems

 

Heliconius erato

Heliconius melpomene

EST results

  

   Number of clones sequenced

1152

1148

   High Quality ESTs1

936 (81%)

1033 (89%)

   Number of unigenes

371

340

   Average sequence length (base pairs)

641

597

BLAST results 2

  

   Unigenes with significant BLAST hits to GenBank protein or nucleotides3 (E-value < 10-5)

257 (69%)

235 (69%)

   Unigenes with significant BLASTX hits to GenBank proteins (E-value < 10-5)

216 (58%)

218 (64%)

   Unigenes with significant BLASTN hits to GenBank nucleotides (E-value < 10-5)

151 (40%)

150 (44%)

SignalP results 2

  

   Unigenes with predicted signal peptides4

86 (24%)

92 (28%)

  1. 1ESTs > 100 bp after trimming raw sequences of vector sequence, low-scoring base calls, and poly-A tails. 2Percentages given in reference to total number of unigenes. 3Includes results from both BLASTN and BLASTX searches. 4We required a positive result from both hidden markov models and neural network methods implemented in SignalP.
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BMC Genomics

ISSN: 1471-2164

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