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Table 2 Bodo-specific hypothetical genes, with evidence of protein domains, transmembrane domains (TMH) signal peptides (SP) and affinities to sequence databases where available.

From: Insights into the genome sequence of a free-living Kinetoplastid: Bodo saltans (Kinetoplastida: Euglenozoa)

Clone

Position

Size (bp)

Interpro match

TMH

SP

Best BLASTp match

Best taxonomic match (% amino acid identity)

14 l 17

3085

645

Oxygenase (IPR005123)

  

Prolyl hydroxylase

Metazoa (53%)

 

16869

1143

Zn-binding protein (IPR001841)

1

   
 

21613

7356

Leucine-rich repeat (IPR001611)

2

   

16 k 02

14573

1125

  

Yes

  
 

16273

1596

  

Yes

GPI-anchored protein

Plantae/Fungi (64%)

 

18275

1212

   

Hypothetical protein

Metazoa (50%)

 

27014

1050

 

7

Yes

  

23 g 24

391

1626

Pleckstrin-like (IPR001849)

    
 

7655

2781

Zinc metallopeptidase (IPR006025)

    
 

21361

23428

   

EF-hand protein

Tetrahymena (47%)

45 a 09

4161

535

Methyltransferase (IPR013216)

    
 

30026

1134

Phosphoribulokinase (IPR006083)

   

Bacteria (50%)

45 a 12

12191

1695

Lipase (IPR008265)

1

   
 

31394

455

Serine-Threonine protein kinase (IPR017442)

  

Protein kinase

Metazoa (46%)

 

41227

264

Nucleotide-sugar transporter (IPR007271)

3

Yes

  

46 a 11

211

753

Endonuclease (IPR001604)

1

Yes

Endonuclease

Bacteria (53%)

 

1166

1416

Thioredoxin (IPR000866)

  

Nucleoredoxin

Plantae (53%)

 

12286

1092

   

Fucosyltransferase

Plantae (36%)

 

14365

765

   

Hypothetical protein

Metazoa (55%)

5 e 15

477

10617

Phosphatidylinositol-4-phosphate kinase (IPR003409)

    
 

14913

1296

Lyzosomal lipase (IPR006693)

1

 

Lipase

Metazoa (59%)

 

16510

2199

Serine endopeptidase (IPR001254)

  

Serine protease

Plantae (46%)

 

21065

546

ABC transporter (IPR013525)

4

Yes

  
 

32153

2613

 

1

   
 

35286

546

Zinc-finger protein (IPR001841)

    

5 m 18

5577

1857

Sodium/hydrogen exchanger (IPR006153)

14

Yes

Sodium/hydrogen exchanger

Metazoa (50%)

 

17069

321

Ankyrin repeat protein (IPR002110)

    

93 d 02

5528

1758

  

Yes

  
 

7529

2589

Chloride channel (IPR0014743)

9

 

Chloride channel

Metazoa (56%)

 

14924

1788

WD40 repeat (IPR001680)

  

Hypothetical protein

Plantae (56%)

93 e 01

56

5505

 

14

   
 

16439

1995

Forkhead associated protein (IPR000253)

    
 

18802

4260

 

8

Yes

  
 

24535

855

Protein tyrosine phosphatase (IPR000340)

  

Protein phosphatase

Plantae (56%)

96 g 09

10627

2559

PDZ protein binding motif (IPR001478)

 

Yes

  
 

16737

1242

 

7

Yes

  
 

26182

948

Leucine-rich repeat kinase (IPR001611)

  

Receptor-type protein kinase

Plantae (54%)

 

27597

483

Leucine-rich repeat kinase (IPR001611)

  

Receptor-type protein kinase

Plantae (34%)