Identification and characterization of ERE1 in Entamoeba sp. A) Reconstruction of Eh_ERE1 consensus sequence from multiple fragmented copies scattered along the E. histolytica assembly. Green boxes, flanking Eh_ERE1 terminal inverted repeats (TIR); black boxes, Eh_ERE1 core region; yellow boxes, single Eh_ERE1 ORF where the arrow indicates sense of transcription; white boxes, E. histolytica scaffolds. Numbers represent coordinates within scaffolds. GenBank accession numbers of scaffolds are indicated on the left. B) Multiple alignment of the consensus protein sequences coded by Eh_ERE1, Ed_ERE1 and Ei_ERE1. Black-shaded letters, identical residues; gray-shaded letters, conservative changes. C) Syntenic regions from E. histolytica (top) and E. dispar (bottom) showing an example of Eh_ERE1 transposition. White boxes, protein coding genes; black box, Eh_ERE1; red boxes, LINEs; gray areas, regions of similarity. GenBank locus tags are indicated above or below genes. Scaffold GenBank accessions are shown on the left. Features on the forward or reverse strand are displayed above or below the scaffolds, respectively.