Identification of mariner-related elements in E. histolytica and E. dispar. A) Phylogenetic position of Eh_mariner, Ed_mariner and Ei_Hydargos (highlighted in blue) in the IS630/Tc1/mariner superfamily. Mariner subfamilies and related transposons (Tc1, ItmD37E, and plant mariner-like elements) are shown. Elements are identified by host name and GeneInfo Identifier (gi). Branches supported by less than 500 bootstrap replicates are depicted as thin black lines; branches having bootstrap values between 500 and 750 are shown as bold grey lines; branches with values above 750 are represented as bold black lines. B) ClustalX alignment of the transposase domain found in Eh_mariner and Ed_mariner together with three closely related transposases. Amino acids conserved in at least 3 sequences are colored in black. Asterisks denote the three conserved glutamic residues typical of this type of transposases. Parentheses indicate number of residues between conserved blocks.