Table 3 Oligonucleotide primers used for RT-PCR
From: Acidic pH shock induces the expressions of a wide range of stress-response genes
Genes | Oligonucleotides | Product length | Genes | Oligonucleotides | Product length |
|---|---|---|---|---|---|
sigR | F: 5'-cgt cta tcc tcc gat tcg agt gag-3' R: 5'-gcc ctc gac gtc cgc cag ata cac-3' | 568 bp | catA | F: 5'-gcc tcc tac cgg cac atg cac gg-3' R: 5'-ctg gtt gcc gtc gac gcg cat gg-3' | 500 bp |
sigH | F: 5'-gtg tcc cag atc gca ggc gag ccc-3' R: 5'-cac gtc ggc ccg gag gag acc ttg-3' | 414 bp | catB | F: 5'-gac gtg gtg cac gcg gcg aag cc-3' R: 5'-gca gcg ggt cgt cgg tga cgt cg-3' | 500 bp |
sigB | F: 5'-gct cac cgt gct gga gga ggg cac-3' R: 5'-ccg atg aag tcg gcg agc gcc gac-3' | 504 bp | sodF | F: 5'-gcc gga ggg gat ccg cca tgt cc-3' R: 5'-ggt gga gcc ctg gcc gac gtt gc-3' | 500 bp |
hrdD | F: 5'-ctt ctc gat ctg gcg gat gcg ctc-3' R: 5'-gtc gag aag ttc gac tac cgc aag g-3' | 519 bp | sodN | F: 5'-ccc gcc tgt ttg ccc cca agg tc-3' R: 5'-cga tgg tgc acc gat cgc cgg-3' | 500 bp |
scoF | F: 5'-gtg ccc gag gtg acg gac gcg-3' R: 5'-tca gac cac gtc ggc cag ctc-3' | 204 bp | trxA | F: 5'-gtg gcc ggc acc ctg aag cat gtg-3' R: 5'-tca gtc ggc gat gaa gtc ctc gag-3' | 333 bp |
dnaK | F: 5'-cgc cag gcg gtc acg aac gtc gac-3' R: 5'-ctg gaa ctg ctt gac cag gta gtc-3' | 480 bp | trxA2 | F: 5'-gtg ccc gag gtg acg gac gcg-3' R: 5'-tca gac cac gtc ggc cag ctc-3' | 315 bp |
dnaJ | F: 5'-gac ggt gag gtt gtc gcc ccg gcg-3' R: 5'-cgc acc ggc ggc gga ccg ggc acc-3' | 504 bp | trxA3 | F: 5'-atg acc agc acc gtg gaa ctc acc-3' R: 5'-cta ctg gcc ttc ctg gcc ttc ctg-3' | 405 bp |
grpE | F: 5'-ccg cgg acc tcc agc ggc tcc agg-3' R: 5'-cct tgg tcc cgg cgt cgt cct ccg-3' | 474 bp | trxB | F: 5'-ctc tac acc gcg cga gca tcc ctg-3' R: 5'-ctt cag gcc cgc aag ctt ctg gtc-3' | 594 bp |
groEL2 | F: 5'-gac gac gtc gcc ggt gac ggt acg-3' R: 5'-cga cag ggc ctc gcc ctc gac gtc-3' | 534 bp | trxB2 | F: 5'-ggc ggt tcg ctc acg acc acg-3' R: 5'-ctc gcc ggt cag ggt gtc acg-3' | 563 bp |