Table 3 Selected qRT-PCR primer sequences and accession numbers
From: Transcriptome profiling of the feeding-to-fasting transition in chicken liver
Gene symbol | Gene name | Ensembl or Gene bank Accession number | Primer sequence (forward/reverse) |
|---|---|---|---|
ACACA | Acetyl-Coenzyme A carboxylase alpha | ENSGALG00000005439 | GAGGAGGGAAGGGAATTAGGAA CCAAGTGGCGGGACTGTT |
ACOX1 | Acyl-Coenzyme A oxidase 1 | ENSGALG00000002159 | TCATCCGGTCTCTGATTGTAGGA GCACTATAGCGGATGGCAATG |
ACLY | ATP citrate lyase | ENSGALG00000003475 | GGCGTGAATGAACTGGCTAAC TAGTCTTGGCATAGTCATAGGTCTGTTG |
CPT1A | Carnitine palmitoyltransferase1A | ENSGALG00000007077 | CCCTGAAAATGCTGCTTTCCTA TGGTGCCTGCAGAAAGTTTG |
CPT2 | Carnitine palmitoyltransferase II | ENSGALG00000010681 | CCTGAACGCCCAGAAACCT CCCTTTTCAAACTGATGAGCAAGT |
CYP7A1 | Cytochrome P450, family 7, subfamily A, polypeptide 1 | ENSGALG00000015432 | TGATGACATGGAAAAAGCAAAGA CCAAAAAGTAGCAGGAATGGTGTT |
EHHADH | Peroxisomal bifunctional enzyme | ENSGALG00000006680 | TCATAGAAAGGAGCGAGAAGC AGCAGGAACCCCAACCAGT |
FADS1 | Fatty acid desaturase 1 | ENSGALG00000007127 | CAGCACCACGCGAAACC TCTACAGAGAGCTTCTTTCCCAAAG |
FADS2 | Fatty acid desaturase 2 | ENSGALG00000007178 | CCATGATCAAGCGCAGGTT ATGTATGTGATGAAATAGCGCATGTAG |
FASN | Fatty acid synthase | ENSGALG00000002747 | TGAAGGACCTTATCGCATTGC GCATGGGAAGCATTTTGTTGT |
HMGCS1 | Hydroxymethylglutaryl-CoA synthase, cytoplasmic | ENSGALG00000014862 | GCTGGTGCTGTTGCTATGCT TGTCTGTCCCCTCTTTTTGC |
HMGCS2 | Hydroxymethylglutaryl-CoA synthase, mitochondrial | ENSGALG00000002960 | GGTGGTGTGTGGGGACAT GGTAGCACTGGATGGAGAGG |
HMGCR | 3-hydroxy-3-methylglutaryl- Coenzyme A reductase | ENSGALG00000014948 | CTGGGTTTGGTTCTTGTTCA ATTCGGTCTCTGCTTGTTCA |
NR1H3 | Nuclear receptor subfamily 1, group H, member 3 (LXRα) | ENSGALG00000008202 | TCCCACTCAACTCAGCACAC CAGACTTCATTTCCCAGCATC |
PCK1 | Phosphoenolpyruvate carboxykinase, cytosolic | ENSGALG00000007636 | CTGCTGGTGTGCCTCTTGTA TTCCCTTGGCTGTCTTTCC |
PPARA | Peroxisome proliferative activated receptor, alpha | ENSGALG00000022985 | AGCATCCAGTCCTTCATCCA AAAAACCCTTACAACCTTCACAA |
SCD | Stearoyl-CoA desaturase(delta-9-desaturase) | ENSGALG00000005739 | TTTGGCAATCGGCCGTAT TGGTAGTTGTGGAAACCTTCTCCTA |
SREBF1 | Sterol regulatory element binding protein 1 | gb:AY029224 | GTCGGCGATCCTGAGGAA CTCTTCTGCACGGCCATCTT |
SREBF2 | Sterol regulatory element binding protein 2 | ENSGALG00000011916 | GGCTGGCTTCTCCCCCTAT GTTCATCCTTAACCTTTGCATCAT |