Table 2 Microsatellite markers' characteristics and genetic variability
Marker | Primer pairs | E.S | Tm | Samples | N | na | ne | Ho | He | HWEG2 |
|---|---|---|---|---|---|---|---|---|---|---|
Boms2 | F: GCTGTTTTGAATGTCAGCATC R: TGTATGCGTGACTGTTTACG | 128 | 50 | wild laboratory | 20 13 | 3 3 | 1.75 2.15 | 0.45 0.54 | 0.44 0.56 | - - |
Boms3a | F: CAGTCGCCCTTTAATTTGC R: GGGTCCTTTTGTTCTCAGG | 176 | 50 | laboratory | 32 | 4 | 2.39 | 0.63 | 0.59 | - |
Boms3b | F: AGGACCCTGGCACAATTCG R: TATGGCATGGCAAGCAGC | 171 | 50 | laboratory | 32 | 4 | 2.26 | 0.59 | 0.57 | - |
Boms5 | F: TCTCGCCCCAATTACCAC R: GAATTTTGGCAACATCCAAGC | 105 | 50 | laboratory | 34 | 4 | 3.25 | 0.79 | 0.70 | - |
Boms6a | F: TCACTAAAAGGAGTCCGCAC R: GAGCAGGTCAGAGGCAAAAG | 173 | 50 | laboratory | 35 | 3 | 1.64 | 0.43 | 0.39 | - |
Boms6b | F: AAACCTTACCCTTTTGCCTC R: AGTGCCAACTGAATGCTG | 118 | 50 | laboratory | 35 | 3 | 2.10 | 0.49 | 0.53 | - |
Boms8 | F: TGACATACATGCCTTCATTCAC R: CAGAAAAGCTTAAAACTAGCGG | 75 | 50 | wild laboratory | 20 9 | 6 3 | 3.10 2.66 | 0.85 0.56 | 0.69 0.66 | - - |
Boms10 | F: CAGAGCATCTCGCTTTGG R: TCAACAATCCCAGCAAAATC | 172 | 50 | wild laboratory | 20 33 | 5 2 | 3.33 1.27 | 0.70 0.12 | 0.72 0.22 | - + |
Boms11 | F: ATAGGCATTGGCAGCGAAG R: CACAGTGGGCCGAAATCAC | 185 | 50 | wild laboratory | 20 25 | 4 2 | 1.88 1.68 | 0.50 0.32 | 0.48 0.41 | - - |
Boms12 | F: CGCGTTTTCATACTTTAACACC R: TTCATTTGGCCTTTGTGC | 158 | 50 | wild | 19 | 3 | 1.17 | 0.16 | 0.15 | - |
Boms14 | F: TTTGTAATTCGCAGAAGGCAC R: AGGAGGACTGACAGAAGGACAC | 147 | 50 | wild | 8 | 4 | 2.72 | 0.38 | 0.68 | Â |
Boms16 | F: CAGACAATGGATGGATACATGC R: GGAGAAGTCAAATTGTGACAGC | 109 | 50 | wild | 20 | 5 | 1.72 | 0.50 | 0.43 | - |
Boms17 | F: ATTAGACCATAGTGTTCTCAC R: AAGATGTTGAGTGCCGTTG | 170 | 50 | wild laboratory | 20 31 | 7 5 | 5.76 1.41 | 0.35 0.19 | 0.85 0.29 | + - |
Boms18 | F: GCCATGAATGCAGACCAC R: CCTATTCAAATGCACGCAAAAC | 171 | 50 | wild laboratory | 20 33 | 6 3 | 2.56 1.94 | 0.80 0.39 | 0.63 0.49 | - + |
Boms21 | F: TCGCCTCTTACCTCACAACC R: ACCATCCTTAGTCAGCACAGTC | 188 | 50 | wild laboratory | 20 28 | 6 4 | 3.77 1.16 | 0.75 0.14 | 0.75 0.14 | - - |
Boms22 | F: GTAAAGCACACGGAAGCG R: TGAGGTCAAAAAGGATGCTAAG | 211 | 50 | wild laboratory | 18 7 | 2 2 | 1.06 1.32 | 0.06 0.29 | 0.06 0.26 | - - |
Boms24 | F: ATTTCGCTTGCCACAAAC R: CGCCCAAGCACTTAAAAC | 215 | 50 | laboratory | 33 | 2 | 1.77 | 0.39 | 0.44 | - |
Boms25 | F: TGGAATGCGCTATTTTGTTG R: ACTCGTATATACGTACATGG | 167 | 50 | wild laboratory | 20 33 | 5 3 | 3.49 2.16 | 0.80 0.55 | 0.73 0.55 | - - |
Boms27 | F: CGACTTGAAGGACAATTGG R: GGCGTGAGTAGTTTCTATAAGC | 129 | 50 | wild laboratory | 20 10 | 5 3 | 2.02 2.41 | 0.55 0.50 | 0.52 0.62 | - - |
Boms29 | TGAAGGTGATGAATGAAAGC GGAATGACTGTGAGCAAGC | 118 | 50 | wild laboratory | 20 13 | 5 1 | 2.57 1.00 | 0.60 0.00 | 0.63 0.00 | - |
Boms30 | F: CTGACTTCTTGCTTTACACG R: CAGCTTATCTGCTTTAAGTGC | 150 | 50 | wild laboratory | 20 9 | 4 4 | 2.12 2.19 | 0.70 0.78 | 0.54 0.58 | - |
Boms31 | F: TGCTTGAGTTGCTCGTTGG R: GCCGCATGACATAAAGAATCG | 173 | 50 | wild laboratory | 20 30 | 4 2 | 3.27 1.03 | 0.75 0.03 | 0.71 0.03 | - |
Boms32 | F: TGTATGTATTTGTGCGTCG R: GCTTAGACCATTTGCTCC | 125 | 50 | wild | 20 | 7 | 3.96 | 0.55 | 0.77 | - |
Boms34 | F: ACGCCGCACACTTCTTAAAC R: CACCCAACTTTTGTAGTTTCC | 219 | 50 | laboratory | 34 | 3 | 2.08 | 0.65 | 0.53 | - |
Boms47 | F: CAAACACACGCTAAAACG R: TTTAACCCAGAGGCTTGC | 158 | 50 | wild | 18 | 6 | 3.15 | 0.61 | 0.70 | - |
Boms58 | F: AGTTGGACGCGCACATATC R: AGCGCGTACGAGCTTTAGC | 181 | 50 | wild laboratory | 18 30 | 7 3 | 5.02 1.15 | 0.72 0.13 | 0.82 0.13 | - - |
Boms59 | F: AGCGCTTACATAAATATAGCTAC R: TCCCCGTAAAGCCATAAAGTC | 171 | 50 | wild | 20 | 5 | 2.27 | 0.50 | 0.57 | - |
Boms60 | F: TGGACGCGCACATATCAG R: ACGACGTTTAGCGGAAATGAG | 170 | 50 | wild laboratory | 20 37 | 6 3 | 3.14 1.21 | 0.70 0.19 | 0.70 0.18 | - - |
Boms62 | F: CTTTCGCTGCCTCCATTTG R: CAAAACCCCTCTGCAATCC | 174 | 50 | wild | 20 | 2 | 2.00 | 0.55 | 0.51 | - |
Boms64a | F: TGCTAGGCTGAACATTCG R: TGTTTTGCTGTTTCCAGG | 129 | 50 | wild | 20 | Multiple bands | Â | |||
Boms64b | F: TGGAAACAGCAAAACACC R: AGCGAATCAAGAGACAGC | 137 | 50 | wild | 10 | Multiple bands | Â | |||
Medflymic9 | Stratikopoulos et al., submitted | 50 | wild | 8 | 3 | 2.42 | 0.88 | 0.63 | Â | |
Medflymic142 | >> | 50 | wild | 9 | monomorphic | Â | ||||
Medflymic149 | >> | 50 | wild | 8 | monomorphic | Â | ||||
Medflymic150 | >> | 50 | wild | 8 | 3 | 2.03 | 0.50 | 0.54 | Â | |
Medflymic151 | >> | 50 | wild | 20 | 5 | 2.66 | 0.35 | 0.64 | + | |
Medflymic152 | >> | 50 | wild | 20 | 6 | 5.00 | 0.40 | 0.82 | + | |
Medflymic153 | >> | 50 | wild | 20 | 3 | 2.24 | 0.40 | 0.57 | - | |
Medflymic154 | >> | 50 | wild | 10 | monomorphic | Â | ||||
Medflymic157 | >> | 50 | wild | 10 | monomorphic | Â | ||||
Medflymic158 | >> | 50 | wild | 8 | 3 | 2.25 | 0.63 | 0.59 | Â | |
Medflymic163 | >> | 50 | wild | 10 | monomorphic | Â | ||||
Medflymic22 | >> | 55 | wild | 20 | Multiple bands | Â | ||||
Medflymic23 | >> | 55 | wild | 20 | monomorphic | Â | ||||
Medflymic29 | >> | 55 | wild | 20 | Multiple bands | Â | ||||
Medflymic40 | >> | 55 | wild | 20 | Multiple bands | Â | ||||
Medflymic61 | >> | 55 | wild | 20 | 4 | 2.32 | 0.45 | 0.58 | - | |
Medflymic64 | >> | 55 | wild | 20 | monomorphic | Â | ||||
Medflymic72 | >> | 55 | wild | 20 | monomorphic | Â | ||||
Medflymic109 | >> | 55 | wild | 20 | 2 | 1.05 | 0.05 | 0.05 | - | |
| Â | Mean wild (monomorphic excluded) Mean laboratory (monomorphic excluded) | Â | 19.5 26.5 | 4.63 3.14 | 2.74 1.90 | 0.53 0.44 | 0.57 0.44 | Â | ||