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Table 5 Accession numbers and SSRs for GenBank deposited sequences http://www.ncbi.nlm.nih.gov/ amplified from 16 Phytophthora species (Table 1) using primers designed on P. infestans with the first approach (Fig. 1A).

From: Use of genome sequence data in the design and testing of SSR markers for Phytophthora species

Phytophthora
species
SELECTED PRIMERS(a)
  I3F-4R I5F-I6R I7F-I8R I9F-I10R I11F-I12R I13F-I14R
P. alni subsp. alni
SCRP2
NS++
58°C/1.7 mM*
EF216535
No SSR;
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216513
(aag)4
(agg)6
58°C/1.0 mM*
NA+ EF216477
(agg)4
(aag)5
58°C/1.7 mM*
P. cambivora
SCRP67
NS++
58°C/1.7 mM*
NA+ NS++
58°C/1.7 mM*
EF216516
(acg)4
(aag)5
(agg)6
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216478
(agg)5
(aag)5
58°C
1.7 mM*
P. cinnamomi
SCRP115
NS++
58°C/1.7 mM*
NS++
58°C/1.7 mM*
NS++
58°C/1.7 mM*
EF216509
(aag)14
58°C/1.0 mM*
EF216494
(aag)4
58°C/1.7 mM*
NS++
58°C/1.7 mM*
P. citricola
SCRP130
NS++
58°C/1.7 mM*
EF216534
(agc)4; (agc)5
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216520
(aag)4
58°C/1.0 mM*
EF216498
(aag)4
58°C/1.7 mM*
EF216482
(agg)9
(aag)5
58°C/1.7 mM
P. europaea
SCRP622
NS++
58°C/1.7 mM*
NS++
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216512
No SSR
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216476
(agg)5
(aag)5
58°C/1.7 mM*
P. fragariae
var. rubi
SCRP333
NS++
58°C/1.7 mM*
NA+ NA+ EF216500
(acg)4
58°C/1.0 mM*
NA+ NA+
P. ilicis
SCRP377
NS++
58°C/1.7 mM*
EF216532
(agc)9
58°C/1.0 mM*
NA+ EF216501
No SSR
58°C/1.0 mM*
EF216495
No SSR
58°C/1.7 mM*
EF216483
(aag)4
(agc)4
58°C/1.7 mM
P. infestans
sc 03.26.3.3
NS++
58°C
1.7 mM*
EF216524
(agc)6
(agc)5
58°C/1.0 mM
NS++
58°C/1.7 mM*
EF216499
(aag)11
58°C/1.0 mM*
EF216487
(acc)8
58°C/1.7 mM*
EF216474
(aag)7
(aag)4
58°C/1.7 mM
P. inundata
SCRP644
NS++
58°C/1.7 mM*
NS++
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216508
No SSR
58°C/1.0 mM*
EF216497
No SSR
58°C/1.7 mM*
NA+
P. lateralis
SCRP390
NS++
58°C/1.7 mM*
EF216527
(agc)4
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216507
No SSR
58°C/1.0 mM*
EF216493
No SSR
58°C/1.7 mM*
EF216481
(agg)5
(aag)4
58°C/1.7 mM*
P. nemorosa
SCRP910
NS++
58°C/1.7 mM*
EF216531
(agc)7
58°C/1.0 mM*
NA+ EF216503
No SSR
58°C/1.0 mM*
EF216492
(aag)4
58°C/1.7 mM*
EF216486
(acg)4
(aag)4
(agc)4
58°C/1.7 mM*
P. pseudosyringae
SCRP674
NS++
58°C/1.7 mM*
EF216529
(agc)7
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216502
No SSR
58°C/1.0 mM*
EF216496
(aag)5
58°C/1.7 mM*
EF216485
(acg)4
(aag)4
58°C/1.7 mM*
P. psychrophila
SCRP630
NS++
58°C/1.7 mM*
EF216528
(agc)4
58°C/1.0 mM*
NS++
58°C/1.7 mM*
NA+ EF216491
(aag)4
58°C/1.7 mM*
EF216484
(aag)4
(agc)4
58°C/1.7 mM*
P. quercina
SCRP541
NS++
58°C/1.7 mM*
NS++
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216506
No SSR
58°C/1.0 mM*
EF216490
(aag)5
58°C/1.7 mM*
EF216480
(agg)6
(aag)4
58°C/1.7 mM*
P. ramorum
SCRP911
NS++
58°C/1.7 mM*
EF216525
No SSR
58°C/1.0 mM*
NS++
58°C/1.7 mM*
EF216505
No SSR
58°C/1.0 mM*
EF216489
(acc)4
58°C/1.7 mM*
EF216479
(aac)7
(agg)9
58°C/1.7 mM
P. sojae
SCRP555
NS++
58°C/1.7 mM*
EF216526
No SSR
58°C/1.0 mM*
NA+ EF216504
(agc)4
58°C/1.0 mM*
EF216488
No SSR
58°C/1.7 mM*
EF216475
(agg)7
(aag)5
58°C/1.7 mM*
  1. (a) = Primers listed in Table 2 and not reported in this table are those that did not produce any reliable sequence (see text).
  2. NA+ = Isolate-primer combinations that did not produce any amplification or produced complex profiles (two or more fragments).
  3. NS++ = Isolate-primer combinations for which single PCR bands were obtained but direct sequencing did not produce reliable results.
  4. °C/mM* = Selected annealing temperature (°C) and MgCl2 concentration (mM) in PCR reactions.