Expression profiles of PLS1, TSP3 , TPL1 in different tissues from M. grisea. PLS1, TSP3, TPL1 expressions were quantified by real-time RT-PCR using RNA extracted from mycelia (M), spores (S), 24-h old appressoria (A) from P1.2 wild type strain, perithecia (P) from crosses between P1.2 (MAT1.2) and TH12 (MAT1.1) strains and 3-days old barley leaves infected by P1.2 (I). PLS1, TSP3, TPL1 expressions were calculated relative to the transcripts levels of the constitutively expressed gene ILV5 (MGG_01808.5) according to the formulae: 2-ΔCt = 2-(CtgeneX-CtILV 5). Each data point is the average of three biological replicates. Standard deviation is indicated by error bars. PLS1 is expressed in all tissues whereas TSP3 and TPL1 are expressed in mycelia and perithecia.